Extracellular Vesicles

Unlocking the potential of Extracellular Vesicles for groundbreaking research and therapeutic applications.

At OBBI, we focus on advancing extracellular vesicle technologies to drive innovation in research, diagnostics, and therapeutic development.

What are extracellular vesicles ?

Extracellular vesicles are nano-sized particles that are delimited by a lipid bilayer and that play a crucial role in intercellular communication. These vesicles exhibit variability in size and content, and their involvement in tissue homeostasis and pathologies is well-documented. EVs facilitate the transport of biomolecules, including proteins, lipids, nucleic acids and organelles to neighbouring cells and distant sites. The significance of these vesicles has led to their exploration in fields such as regenerative medicine and biomedical research, where their potential applications in therapeutic interventions and diagnostics are being investigated. Current research is directed towards investigating their capacity to deliver native bioactive molecules, or to function as vehicles for drug delivery and as biomarkers in multifaceted roles.

Services

Advanced development and optimization of extracellular vesicles bioproduction:
Ensuring a robust, standardized, and scalable extracellular vesicle bioproduction process is key to industrial and clinical applications. At OBBI, we integrate cutting-edge technologies to optimize every step of its production, isolation and characterization, ensuring high quality, purity, and functionality.

Cell sourcing and expansion

Diverse cell sources for extracellular vesicle production, including mesenchymal stromal cells, and other specific cell types, on-demand.
Controlled cell expansion under GLP-like environments to ensure reliability.
2D and 3D expansion of cells to maximize extracellular vesicle yields while maintaining cell viability and stability.

EV Production Optimization

Development of optimized culture conditions to enhance EV high yield production.
Use of serum-free and chemically defined culture media to eliminate external contaminants.
Tailored production methods to match the specific applications of EVs, including cell priming or specific culture conditions.

EV isolation, characterization and quality controls

Implementation of standard isolation techniques, such as differential ultracentrifugation, size exclusion chromatography, automatic nanofiltration, and tangential flow filtration.
Rigorous characterization protocols aligned with international standards (e.g., MISEV guidelines) to ensure identity, purity, and functionality.
Application of advanced analysis technologies, including nanoparticle tracking analysis (for size, concentration, zeta potential, colocalization), nanoflow cytometry for single EV characterization, super-resolution microscopy for single molecule visualization on single EV.
Access to complementary analysis (cryo-TEM, transcriptomics, proteomics,…)

EV engineering

Surface modification of EVs for plasma stabilization or cell targeting applications.
Association of exogeneous therapeutic molecules using post-production engineering methods.
Quantification of engineering efficiency using bulk and single particle methods.

EV storage and traceability

Development of long-term storage solutions such as cryopreservation, lyophilization, and buffer stabilization to maintain EV integrity/functionality.
Secure storage under temperature-controlled conditions.

applications domains

Extracellular vesicle technology presents several significant market opportunities within the biotech and pharmaceutical industries, driven by its unique ability to mediate intercellular communication and deliver bioactive molecules for a wide range of applications.